EDUCATION
Ph.D., Chemistry - 1993 - 1998, University of Virginia, Charlottesville, VA.
Department of Chemistry (David S. Cafiso, Advisor).
B.A., Chemistry - 1989 - 1993, Ohio Wesleyan, University, Delaware, OH.
Department of Chemistry, Departmental Honors.
EXPERIENCE
Assistant Professor - 2005 - present, Department of Chemistry and Biochemistry, California State University, San Marcos, CA.
Research Associate - 2001 – 2005, University of Southern California, Los Angeles, CA.
Investigated the structure and membrane interaction properties of human amylin, a protein thought to play a major role in the pathogenesis of human type II diabetes (laboratory of Dr. Ralf Langen).
Post Graduate Researcher - 1999 - 2001, University of California, Irvine, CA.
Studied the structural and thermodynamic aspects of peptide–membrane interactions. Developed computer programs for the identification and examination of membrane proteins (laboratory of Professor Stephen White).
Graduate Student - 1993 - 1998, University of Virginia, Charlottesville, VA.
Studied and characterized the membrane interaction of peptides and proteins using a variety of biophysical techniques.
MEMBERSHIPS
The Biophysical Society
Protein Society
American Association for the Advancement of Science
INVITED PRESENTATIONS
Annual Meeting of the Biophysical Society – Membrane Structure and Assembly subgroup symposium.
Title: Energetics, Stability and Prediction of Transmembrane Helices
PUBLICATIONS
JOURNAL ARTICLES
Jayasinghe, S.A., and Langen R., (2005). Lipid Membranes Modulate the Structure of Islet Amyloid Polypeptide, Biochemistry, 44(13):12113-12119.
Jayasinghe, S.A., and Langen R., (2004). Identifying structural features of fibrillar islet amyloid polypeptide using site-directed spin labeling. Journal of Biological Chemistry, 279:48420-48425.
Ellena, J.F., Moulthrop J., Wu, J., Rauch, M., Jayasinghe, S., Castle, J.D., and Cafiso, D.S., (2004). Membrane position of a basic aromatic peptide that sequesters phosphatidylinositol 4,5 bisphosphate determined by site-directed spin labeling and high-resolution NMR. Biophysical Journal, 87:3221-3233.
Isas, J.M., Patel, D.R., Jao, C., Jayasinghe, S., Cartailler, J.P., Haigler, H.T., and Langen, R.(2003). Global structural changes in annexin 12- The role of phospholipids, Ca2+, and pH. Journal of Biological Chemistry, 278:30227-30234.
Jayasinghe, S., Hristova, K., and White, S.H. (2001). Transmembrane helix energetics and prediction accuracy. Journal of Molecular Biology, 312:927-934.
Jayasinghe, S., Hristova, K., and White, S.H. (2001). MPtopo: A database of membrane protein topology. Protein Science, 10:455-458.
White, S.H., Ladokhin, A.S., Jayasinghe, S., and Hristova, K. (2001). How membranes shape protein structure. Journal of Biological Chemistry, 276:32395-32398.
Ladokhin, A.S., Jayasinghe, S., and White, S.H. (2000). How to measure and analyze tryptophan fluorescence in membranes properly, and why bother? Analytical Biochemistry, 285:235-245.
Hubbard, C., Singleton, D., Rauch, M., Jayasinghe, S., Cafiso, D., and Castle, D. (2000). The secretory carrier membrane protein family: Structure and membrane topology. Molecular Biology of the Cell, 11:2933-2947.
Jayasinghe, S., Barranger-Mathys, M., Ellena, J.F., Franklin, C., and Cafiso, D.S. (1998). Structural features that modulate the transmembrane migration of a hydrophobic peptide in lipid vesicles. Biophysical Journal, 74:3023-3030.
Franklin, J.C., Ellena, J.F., Jayasinghe, S., Kelsh, L.P., and Cafiso, D.S. (1994). The structure of micelle-associated alamethicin from 1H NMR. Evidence for conformational heterogeneity in a voltage-gated peptide. Biochemistry, 33:4036-4035.
ABSTRACTS
Jayasinghe, S., and Langen R., (2004). Lipid membranes modulate human islet amyloid polypeptide aggregation. Protein Science, 13.
Jayasinghe, S., Apostolidou, M., and Langen, R. (2004). Characterizing Fibrillar Human Islet Amyloid Polypeptide (IAPP). Biophysical Journal, 86.
Jayasinghe, S., Torok, M., and Langen, R. (2003). Interaction of Islet Amyloid Polypeptide with phospholipids vesicles. Biophysical Journal, 84.
Jayasinghe, S., and White, S.H. (2001). Partitioning of peptides at the bilayer interface: The role of helicity and pattern of hydrophobicity. Biophysical Journal, 80:A2431.
Jayasinghe, S., Sacha, S., Castle, J.D., and Cafiso, D.S. (2000). Phosphoinositide interactions of a peptide from a secretory carrier membrane protein. Biophysical Journal, 78:A2439.
Jayasinghe, S., Hristova, K., and White, S.H. (2000). MPEx and MPtopo: Tools for membrane protein topology analysis. Biophysical Journal, 78:A930.
Frazier, A., Hinderliter, A., Jayasinghe, S., Almeida, P., Biltonen, R., Creutz, C., and Cafiso, D.(1999). Investigation of the influence of basic peptides on acidic lipid rearrangement in vesicles. Biophysical Journal, 76:A213.