A new method has been developed to measure the specific rotation of optically active compounds using flow injection as a sample introduction system. The laser-based Polarimetry for the detection and identification of chiral molecules was demonstrated by a study of the antibiotic: gentamicin. In addition, the determination of the specific rotation of several different amino acids and the corresponding peptides under different conditions of pH has been accomplished. These results have shown that specific rotation measurements can be used to monitor subtle changes in the structure of a complicated molecule. The use of laser-based polarimeter as a selective detector for optically active compounds has been extended to the detection of achiral compounds.

Postdoctoral Training:  Department of Pharmacology, Meharry Medical College, Nasville, TN.

Doctor of Philosophy:  Analytical Chemistry, University of Arkansas, Fayetteville, AR.

M.B.A.: Management, Tennessee State University, Nashville, TN.

Bachelor of Science (ACS Professional Degree), Summa Cum Laude: Chemistry, Southwestern Oklahoma State University, Weatherford, OK.

2015   (e-Portfolio) Karno Ng "Design of a Fully On-line Introduction General Education Science course with a Hands-on Lab Component." https://contentbuilder.merlot.org/toolkit/users/babe/sanmarcos

2015   Karno Ng, "Development of an On-line General Education Introductory Science Course with a Hands-on Lab Component for Non-science Majors."  Journal of Chemical Education.  2015, 20, 1-7.

2011   Todd A. Astorino, Brian J. Martin, Lena Schachtsiek, Keau Wong & Karno Ng.  “Minimal Effect of Acute Caffeine Ingestion on Intense Resistance Training Performance.” Journal of Strength and Conditioning Research. 2011 25(6) 1752-1758.

2003   Karno Ng and Sean W. Linder, "HPLC Separation of Tetracycline Analogs: Comparison Study of Laser-based Polarimetric Detection with UV Detection," Journal of Chromatographic Science. 2003, 41, 460-466.

2002   William C. Alston, Karno Ng, "Rapid Colorimetric Screening Test for y-hydroxybutyric acid (Liquid X) in Human Urine," Forensic Science International. 2002, 126, 114-117.

2001   Karno Ng, Ratnasamy Somanathan and Patrick J. Walsh, "Synthesis of Chiral Resolved Pentadentate Sulfonamide-Based Ligands," Tetrahedron Asymmetry, 2001, 12, 1710- 1722.

1999   Karno Ng, Thomas J. Edkins and Donald R. Bobbitt, "Direct Specific Rotation Measurement of Amino Acids, Dipeptides and Tripeptides by Laser-Based Polarimetry," Chirality, 1999, 11, 187-194.

1992   Donald R. Bobbitt and Karno Ng, "Polarization Selective Detection of Biomolecules," Proc. Int. Conf. Lasers (1992), Volume Date 1991, 14th, 808-15.

1992   Donald R. Bobbitt and Karno Ng, "Chromatographic Analysis of Antibiotics in Food Materials, " J. Chromatogr., 1992, 624,153-170.

1992   Donald R. Bobbitt, Faye K. Ogasawara, Yunlong Wang and Karno Ng, "Renewable Optical Fiber Sensors Based on a Dynamically Modified Surface Interaction," SPIE Vol. 1648 Fiber Optic Medical and Fluorescent Sensors and Applications (1992), 227-237.

1991   Karno Ng, Patrick D. Rice, and Donald R. Bobbitt, "Identification and Quantitation of Gentamicin in Milk Using HPLC Separation and Laser-Based Polarimetric Detection," Microchemical Journal., 1991, 44, 25-33.

Conferences & Presentations

Nov. 7, 2015: The 45th Western Regional Meeting of the American Chemical Society. San Marcos, CA . Division of Analytical Chemistry.  Karno Ng.  “Development of separation and detection method for chemotherapeutic drugs”.

Nov. 7, 2015: The 45th Western Regional Meeting of the American Chemical Society.  San Marcos, CA. Division of Chemical Education.  Karno Ng. ” Using technology to reach out to new generation for a fully online chemistry course.”

April 11, 2015: Southern California Conference For Undergraduate Research, San Diego, CA. Ting-An Lim, Elizabeth Nicolas and Karno Ng. ” Study of the Equilibrium Reaction of Ferroin and Ferriin.”

March 14, 2015: The 18th Annual CSU Symposium on University Teaching, CSU Los Angeles, Karno Ng. “"Reach out and Connect to the New Generation with Technology."

Dec. 2, 2014: Student Poster Showcase, CSU San Marcos, Tyler Jackson and Karno Ng, “Design and Application of an Automated Reaction System For Everyday Life Products.”

May 7, 2014: 2st Chemistry & Biochemistry Celebration, CSU San Marcos,  Matt Mathew Madden & Karno Ng, “Develop an Extraction Method for Mitragynine."

April 12, 2014: Southern California Conference For Undergraduate Research, Irvine, CA. Yescenia Perez and Karno Ng. “Development of Separation and Detection Method for Phenytoin and Chemotherapeutic Drugs.”

April 12, 2014: Southern California Conference For Undergraduate Research, Irvine, CA. Tyler Jackson and Karno Ng. “Design of an Automated and Dynamic Acid-base Reaction Visual Display for  Enhancement of Scientific Interest Among General Public."
 
April 11, 2014: Celebration of Faculty Scholarship and Creative Activity, CSU. “Detection of Common Components in  Coffee and Chocolate and  its Application to Biological Samples." Karno Ng.

April 26, 2013: Teaching Exposition, California State University San Marcos, “Poll Everywhere, A New Way to Click.”

 April 23, 2012: Faculty Center "Teaching Science: Engaging Students Digital/Electronic Solutions" Workshop, California State University San Marcos, Karno Ng, "Teaching Chemistry On-line".

Jan. 8, 2011: 21st Annual CSU Biotechnology Symposium, Anaheim, CA. Karno Ng, Ngoc Tong, Natalia Vladi. "Development of an Effective HPLC Separation and Detection Schemes for Tetracycline Analogs with UV and Evaluation of Laser-Based Polarimetry as an Alternative and/ or Complimentary Detection Method for Tetracycline Analogs".

Apr. 1, 2009: San Diego Science Festival, California State University, San Marcos, CA. Karno Ng & Judit Hersko , "The Art of Chemistry".

Jan. 15, 2009: 21st Annual CSU Biotechnology Symposium, Los Angeles, CA. Luis Thompson and Karno Ng. "Development of a Sensitive and Efficient Extraction and Detection Method for Theophylline----a Commonly Prescribed Medicine for Asthma and Bronchitis patients."

Nov. 20, 2008: Southern California Conference For Undergraduate Research, Pomona, CA. Misa Le and Karno Ng. "Development of a Sensitive and Efficient Extraction and Detection Method for Caffeine and Paraxanthine---- a major metabolite of caffeine".

Nov. 20, 2008: Southern California Conference For Undergraduate Research, Pomona, CA. Luis Thompson and Karno Ng. "Development of a Sensitive and Efficient Extraction and Detection Method for Theophylline----a Commonly Prescribed Medicine for Asthma and Bronchitis patients."

Apr. 17, 2008: Southern California Undergraduate Research Conference, University of California, Riverside, CA. Misa Le, Luis Thompson and Karno Ng. "Development of a sensitive detection method for Theophylline - a commonly prescribed medicine for asthma and bronchitis patients."

Apr. 17, 2008: Southern California Undergraduate Research Conference, University of California, Riverside, CA. Anthony Rowley and Karno Ng. "Development of Learning Modules for Chemistry and Art Courses: Investigation of Chemical Equilibrium and Le Chatelier's Principle".

Apr. 12, 2008: 11th CSU Symposium on Universal Teaching, Cal Poly Pomona, Pomona, CA. Karno Ng. "Teaching with Technology: Video-conferencing between Future Educators and 6th Graders."

Apr. 8, 2006: 11th annual Maria Goeppert-Mayer Interdisciplinary Symposium. San Diego, CA. Sarah F. Roche, Adrian A. Atherton, Karno Ng and William G. Tong. "Laser wave mixing-based detection of trace metals entrapped in Sol-Gel."

Oct. 10, 2002: Southern California Undergraduate Research Conference in Chemistry & Biochemistry, Northridge, CA. Robert Quitania and Karno Ng, "Separation of drugs with interaction: Phenytoin and Dexamethasone by High Performance Liquid Chromatography."

Apr. 20, 2001: San Diego and North County Chemistry Faculty Meeting, San Marcos, CA. Karno Ng, "Innovative teaching and course structure in Quantitative Analysis."

Mar. 21-25, 1999: American Chemical Society National Meeting, Anaheim, CA. Karno Ng and Donald R. Bobbitt, "Specific Rotation Measurement of Optical Active Compounds via Flow Injection Method."

Mar. 24-20, 1996: American Chemical Society meeting, New Orleans, Louisiana. Karno Ng and Donald R. Bobbitt, "Direct Specific Rotation Measurement of Amino Acids, Dipeptides and Tripeptides by Laser-Based Polarimetry."

Oct. 2-7, 1994: Federation of Analytical Chemistry and Spectroscopy Society Meeting, St. Louis, Missouri.  Karno Ng, T.J. Edkins and D.R. Bobbitt, "Development and Application of New Techniques for Probing Chiral Species Using Polarized Light."

Oct. 17-22, 1993: Federation of Analytical Chemistry and Spectroscopy Society Meeting, Detroit, Michigan.  Karno Ng and Dr. Donald R. Bobbitt,  "Ultrasensitive Measurement of Specific Rotation via Laser-Based Polarimetry: Application to the Study of Complex Bimolecular Structure."

Sept. 20-25, 1992: Federation of Analytical Chemistry and Spectroscopy Society Meeting, Philadelphia PA.  D.R. Bobbitt and Karno Ng, "Polarization-Selective Spectroscopy: New Techniques for Studying Complex Bimolecular Structure."

Oct. 7-12, 1990: Federation of Analytical Chemistry and Spectroscopy Societies, Cleveland OH.  Patrick D. Rice, Karno Ng, Faye Ogasaware and Donald R. Bobbitt, "Laser-Based Polarimetric Detection of Gentamicin in Milk."